Extended Data Fig. 9: TH+ neurons are susceptible to damage from 2C cells after treatment with LPS and IFNγ.
From: Intestinal infection triggers Parkinson’s disease-like symptoms in Pink1−/− mice
Quantification (left) and representative images (right) of the cytotoxic assay in which neurons from Pink1−/− mice were treated with LPS, IFNγ or left untreated (control) and co-cultured with or without OGDH-specific CD8+ T cells isolated from 2C mice for two days. Exogenous addition of OGDH peptides (last column) was used as a positive control. Dopaminergic neurons were identified by TH staining, and damage was determined by the presence of swollen cell bodies and the shortening of dendrites. Data represent measurements from individual coverslips and are representative of three independent experiments. P values were determined by one-way ANOVA at a 95% confidence interval with Tukey’s multiple comparison’s test. Data are mean ± s.e.m.
