Extended Data Fig. 2: C. rodentium induces mild colonic pathology and inflammation in both wild-type and Pink1−/− mice.
From: Intestinal infection triggers Parkinson’s disease-like symptoms in Pink1−/− mice
a, The distal colon was collected at 13 d.p.i. and assessed for colon-associated C. rodentium in infected wild-type and Pink1−/− mice via serial dilution and plating on MacConkey agar. Data representative of two independent experiments. b, Analysis of colonic epithelial hyperplasia via measurement of crypt heights from haematoxylin and eosin (H&E)-stained colon sections. Data represents individual crypt measurements from five wild-type mice and six Pink1−/− mice. Data representative of two independent experiments. c, Tissue pathology scores from H&E-stained distal colon sections from infected wild-type and Pink1−/− mice. d, e, Protein quantification of faecal S100A8/A9 (d) and lipocalin-2 (e) from control and infected wild-type and Pink1−/− mice. Data pooled from two independent experiments. P values were determined by two-tailed Mann–Whitney unpaired t-test at a 95% confidence interval (a, b), two-way ANOVA at a 95% confidence interval (c), or two-way ANOVA at a 95% confidence interval with Tukey’s multiple comparison’s test. Data are mean ± s.e.m.
