Extended Data Fig. 1: Loss of LLG1 phenocopies fer in flg22-triggered formation of the FLS2–BAK1 complex, and ROS production.
From: Mechanisms of RALF peptide perception by a heterotypic receptor complex
a, Tissue-specific expression patterns of FER (AT3G51550), LLG1 (AT5G56170), LLG2 (AT2G20700) and LLG3 (AT4G28280) were obtained using Genevestigator (www.genevestigator.com) and are based on the ‘AT_mRNASeq_ARABI_GL-1’ dataset. FER is ubiquitously expressed throughout most tissues, including leaves. Similarly, LLG1 shows strong expression in vegetative tissue—unlike LLG2 and LLG3, which are predominantly expressed in reproductive tissue. All assays in this study were performed using seedlings or leaf tissue. LLG1, LLG2 and LLG3 expression in these tissues is highlighted by a black box. b, Loss of LLG1 results in reduced flg22-triggered ROS production. ROS burst was measured in leaf discs of the indicated genotypes after elicitation with 100 nM flg22. Values are represented as total photon counts over 40 min. Biologically independent leaf discs, n = 8 for all genotypes. Values shown as mean ± s.e.m. Letters indicate the result of a post hoc Tukey test. Columns with same letters are statistically indistinguishable (>95% confidence); see ‘Supplementary statistical information’ in Supplementary Information for multiple-comparison P values. The experiments were repeated at least three times with similar results. c, LLG1 contributes to flg22-induced formation of the FLS2–BAK1 complex. Arabidopsis Col-0, fer-2 or llg1-2 seedlings were treated with 100 nM flg22 for 10 min before immunoprecipitating FLS2. Western blots for protein detection were probed with anti-FLS2 and anti-BAK1 antibodies. The experiments were repeated at least three times with similar results. See Supplementary Fig. 1 for gel source data.
