Extended Data Fig. 9: Genome-wide analysis of gene expression of SadCas9-2×VP64-treated dy^2j/dy^2j mice.

a–c, Differential expression analysis derived from RNA-sequencing results from the quadriceps of treated and untreated mice (n = 3 per group). a, b, No guide: dy^2j/dy^2j mice injected with AAV9 carrying only the SadCas9–2×VP64 at the dose of 3 × 10¹¹ viral genomes per gram of mouse. a, c, Three guides: dy^2j/dy^2j mice injected with AAV9 carrying the SadCas9–2×VP64 and three guides at the dose of 3 × 10¹¹ viral genomes per gram of mouse (total dose of 2 × 3 × 10¹¹ viral genomes per gram of mouse). b, c, Untreated: dy^2j/dy^2j mice that had not been injected with AAV9. False discovery and multiple testing were controlled for and an adjusted P value was computed using the Benjamini–Hochberg method. Differentially expressed genes were defined as genes with a more than twofold change and adjusted P < 0.05. For each comparison, significantly differentially expressed genes (false-discovery rate-adjusted P < 0.05) are coloured. Red data points indicate a log-transformed fold change in expression greater than one; blue data points indicate a log-transformed fold change in expression less than one. d, Hierarchical clustering was performed on the normalized counts-per-million expression data.