Extended Data Fig. 1: SadCas9–2×VP64 enhances expression of minCMV-driven tdTomato in vitro.
From: A mutation-independent approach for muscular dystrophy via upregulation of a modifier gene
a, b, HEK293T cells were transfected with a plasmid encoding minCMV-driven tdTomato only. c, d, HEK293T cells were transfected with a combination of a plasmid containing minCMV-driven tdTomato and one containing SadCas9–2×VP64 and a sgRNA targeting the minCMV promoter. a, c, Schematic. b, d, Cells were imaged for tdTomato expression by fluorescent microscopy. Bright-field and tdTomato images are shown. The experiment was repeated three times independently with similar results. Scale bars, 50 µm.
