Extended Data Fig. 5: Expression of laminin subunits in tibialis anterior muscles.
From: A mutation-independent approach for muscular dystrophy via upregulation of a modifier gene
Expression of laminin (LM) subunits in tibialis anterior muscles of wild-type, dy2j/dy2j (intravenously injected with AAVs carrying no guide at 3 × 1011 viral genomes per gram of mouse or three guides at a total dose of 2 × 3× 1011 viral genomes per gram of mouse) and dy2j/dy2j LMα1 (with transgenic overexpression of LMα1) mice (n = 3 for each group). Expression of the LMα1 chain in the tibialis anterior muscle is comparable between dy2j/dy2j mice treated with three guides and transgenic mice. No major differences in expression of LMα2 and LMγ1 were detected between the groups. The LMα4 chain is upregulated in muscles of dy2j/dy2j mice treated with the no-guide AAV and the expression of the LMα4 chain is partially normalized in muscle of dy2j/dy2j mice treated with the three-guide AAV. Scale bar, 50 μm. The experiment was repeated three times independently with similar results.
