Extended Data Fig. 9: YFP and RFP expression for synTF and dCas9–VP64.

a, b, Assay for synTF (a) and dCas9–VP64 (b), corresponding to Fig. 4, as a function of oestradiol (E2) (0–125 nM) and progesterone (Pg) (0–100 nM). YFP fluorescence represents the transcriptional output of either synTF or dCas9–VP64 and RFP fluorescence represents fluorescence of either synTF or dCas9–VP64. Fluorescence was measured at steady state using flow cytometry. Heat maps depict mean fluorescence, and are a representative sample of three biological replicates. The oestradiol dose (31.25 nM) depicted in Fig. 5 is indicated with the black rectangle on the heat maps.