Extended Data Fig. 3: Mass spectra showing glutamylation of E860 of SdeA.
From: Inhibition of bacterial ubiquitin ligases by SidJ–calmodulin catalysed glutamylation
a, Annotated mass spectra for di-glutamylation of E860 of SdeA from samples of immunoprecipitated GFP–SdeA co-expressed with SidJ. b, In vitro glutamylation of SdeA was carried in various conditions (as indicated), followed by ε-NAD+ hydrolysis assays to measure the ubiquitin–ADP ribosylation activity of SdeA. The experiment was repeated three times independently, with similar results.
