Extended Data Fig. 4: In vitro glutamylation of SdeA.
From: Inhibition of bacterial ubiquitin ligases by SidJ–calmodulin catalysed glutamylation
a, Samples of in vitro glutamylation reactions that contained SdeA and SidJ, with or without ATP, were TMT-labelled and analysed by quantitative mass spectrometry. Mono- and di-glutamylation of the catalytic E860 of SdeA was enriched in samples that contained ATP. In vitro glutamylation was performed in n = 3 biologically independent experiments. Samples were labelled with TMT six-plex reagent and analysed in one liquid chromatography–mass spectrometry run. Significant differences between samples were detected by a two-sided Student’s t-test. b, Annotated mass spectra for mono-glutamylation of E860 of SdeA, from SidJ glutamylase in vitro reactions. c, Annotated mass spectra for di-glutamylation of E860 of SdeA, from SidJ glutamylase in vitro reactions.
