Extended Data Fig. 10: TCR repertoire of brain-homing-activated γδ⁺ T cells in newly diagnosed patients with MS.

a, b, Brain homing and activated (CD49d⁺CD29⁺HLA-DR⁺CD38⁺) γδ⁺ T cells were single-cell-sorted from PBMCs of healthy controls (a) and newly diagnosed patients with MS (b). The cells underwent single-cell paired TCR sequencing. Pie charts depicting clonal expansion of γδ⁺ T cells among healthy controls (n = 10) and patients with MS (n = 18). The number of cells with δ chain successfully identified is shown above its pie chart. For each TCR clone expressed by two or more cells (clonally expanded), the absolute number of cells expressing that clone (≥2, ≥5, ≥10, ≥20 and ≥50) is shown by a distinct coloured section. From the single-cell sorted γδ⁺ T cells, RAR-related orphan receptor (ROR) transcripts were amplified using gene-specific primers and sequenced simultaneously with γ and δ chains. c, The number of γδ⁺ T cells positive for the RORC transcript is shown among healthy controls (n = 10) and patients with MS (n = 18). *P = 0.0301, paired t-test. Data are mean ± s.e.m.