Extended Data Fig. 1: Massive clonal expansion of all T cells after EAE immunization.
From: Opposing T cell responses in experimental autoimmune encephalomyelitis
a–e, C57BL/6J mice were immunized for EAE induction, and cells from blood, draining LN, spleen and the CNS were isolated and stained with a cocktail of cell-surface antibodies on different days after immunization (D0 (unimmunized), D3, D5, D7, D10, D12, D15, D17, D19, D21, D23 and D3). a, Infiltrating CD4+, CD8+ and γδ+ T cells were single-cell-sorted on D0 (unimmunized), D7, D10, D15 and D19 after immunization. The cells underwent single-cell paired TCR sequencing. (n = 3 mice per group/time point). In total, we sequenced 1,302 (CD4+), 1,660 (CD8+), and 1,451 (γδ+) paired TCR sequences. b–d, Average percentage clonal expansion of CD4+ (b) CD8+ (c) and γδ+ (d) T cells among unimmunized and immunized mice in all days and tissues combined together. Data are mean ± s.e.m. e, Percentage of identical CD4+, CD8+ and γδ+ TCR sequences shared between blood and the CNS within each day after immunization. f, Frequency of major groups (groups 1–4) of tTγδ17 cells in blood and CNS on different days after immunization. g, Corresponding paired TCRγ and TCRδ sequences that define each major group (groups 1–4) of tTγδ17 cells.
