Extended Data Fig. 2: Functional analysis of lncRNA.
From: Molecular architecture of lineage allocation and tissue organization in early mouse embryo
a, Experimental design for tracking the expression of lncRNAs during in vitro differentiation of mouse embryonic stem cells (mESCs). b, c, Expression of anterior-specific (b) and posterior-specific lncRNAs (c), assayed by real-time PCR, during in vitro differentiation (days 0–6) of mouse embryonic stem cells (n = 3 for KSR and FBS). d, Experimental design for assessing the effect of the loss of lncRNA function on the differentiation of mouse embryonic stem cells in vitro. e, Ablation of lncRNA 3110004A29Rik led to downregulation of mesoderm genes: T, Eomes and Mesp1 at days 5 and 6 of culture (n = 3). f, Ablation of lncRNA RP23-458G12.2 disrupted the expression of mesendoderm genes: Pcdh7, Eomes, Mesp1 and Hand1 at later time points of the seven-day period of differentiation in vitro (n = 3). Gene expression was assayed by real-time PCR. Data are mean ± s.e.m. Significant difference by two-sided t-test; *P < 0.05, **P < 0.01 and ***P < 0.001.
