Extended Data Fig. 5: Functional analysis of Sp1 of the G7 (pre-gastrulation), Hmgb3 of the G1 (late mesoderm/primitive streak) regulon group and Hmga2 of G8 (mesoderm) regulon group on differentiation of the mouse embryonic stem cells.
From: Molecular architecture of lineage allocation and tissue organization in early mouse embryo
a, DNA sequence of two Sp1-knockout cell lines. b, Loss of Sp1 function led to downregulation of the naive marker genes (Rex1, Esrrb and Nanog) and upregulation of formative marker genes (Sall2, Fgf5 and Pou3f1) (n = 3). c, Loss of Hmgb3 function led to upregulation of Cdx2, Cdx4, Hoxa1 and Tbx6 genes related to precursors of the trunk mesoderm and no changes to markers of mesendoerm progenitors. Gene expression was assayed by qPCR. Data are mean ± s.e.m. (n = 3). Significant difference by two-sided t-test; *P < 0.05, **P < 0.01 and ***P < 0.001. d, DNA sequence and sequencing peak map of two Hmga2-knockout cell lines. e, Knockout of Hmga2 led to down regulation of cardiac progenitor marker genes at differentiation day 6 (n = 3). f, Knockout of Hmga2 led to down regulation of cardiomyocyte marker genes at differentiation day 12 (n = 3). g, Immunostaining of embryoid body at day 12 shows lower expression of cardiomyocyte marker c-TnT in Hmga2-knockout cells (scale bars: top, 25 μm; bottom, 5 μm) (n = 3). Data are mean ± s.e.m. Significant difference by two-sided t-test; *P < 0.05, **P < 0.01 and ***P < 0.001.
