Extended Data Fig. 5: Resistant cells are dependent on BORIS for survival.
From: BORIS promotes chromatin regulatory interactions in treatment-resistant cancer cells
a, Dose–response curves to TAE684 in resistant cells expressing control (shCtrl) or BORIS (shBORIS) shRNAs (IC50 values: shCtrl, 537.7 nM; shBORIS, 141.2 nM). Data are mean ± s.d., n = 3 biological replicates. b, Heat map of gene expression values in the same cells as in a (n = 2 biological replicates). Rows are z-scores calculated for each gene in both conditions. c, Immunoblot analysis of the indicated proteins in the same cells as in a. d, e, Immunoblot analysis of the indicated proteins (Cl., cleaved; CC3, cleaved caspase 3) (d), and quantification of trypan blue staining (e) in sensitive and resistant cells expressing control (shCtrl) or BORIS (shBORIS-3 and -4) shRNAs. Data are mean ± s.d., n = 3 biological replicates (*P < 0.05; **P < 0.01; ***P < 0.001; unpaired two-sided t-tests). f–h, Phase-contrast microscopy images (scale bars, 150 μm) (f), growth curves (g) and flow cytometry analyses (h) of propidium iodide (PI) staining in sensitive, intermediate and resistant cells. Data are mean ± s.d., n = 3 biological replicates (***P < 0.0001 for all comparisons; two-way ANOVA). i, qRT–PCR analysis of the expression of the indicated proneural transcription factors in the same sensitive (DMSO) versus MYCNKD and BORISInd (DOX + TAE) cells as in Fig. 1g. Data are mean ± SD, n = 3 biological replicates (*P < 0.05; **P < 0.01; unpaired two-sided t-tests). In c, d, f and h, data are representative of two independent experiments (for gel source data, see Supplementary Fig. 1).
