Extended Data Fig. 6: Structural characterization of human MLL1–NCP complex.
From: Structural basis of nucleosome recognition and modification by MLL methyltransferases
a, Flow chart of cryo-EM data processing of the MLL1–NCP dataset. Electron microscopy map for binding mode 2 was not applied with solvent mask (post-processing) because this procedure severely dampened the electron microscopy density of the WDR5–MLL1–ASH2L regions. b, Representative micrograph of the cryo-EM dataset of the MLL1–NCP complex. c, Representative 2D class averages of cryo-EM particles of the MLL1–NCP complex. d, Angular distribution of particle projections from different binding modes of the MLL1–NCP complex. e, The gold-standard FSC curves calculated between two halves of the MLL1–NCP dataset from different binding modes. f, Local-resolution estimates of the MLL1–NCP complex structure from different binding modes.
