Extended Data Fig. 3: Flow chart of cryo-EM data processing of the MLL1–ubNCP dataset, and overall structural comparison of human MLL1 complex with yeast COMPASS complexes.
From: Structural basis of nucleosome recognition and modification by MLL methyltransferases
a, Flow chart of cryo-EM data processing of the MLL1–ubNCP dataset (resolution of 4.0 Å) that reveals the electron microscopy density of the pre-SPRY domain of ASH2L and the dynamic conformations of H2BK120-conjugated ubiquitin. Masked 3D classifications without realignment were applied to the entire MLL1 complex (left branch), the MLL1–ASH2L region (middle branch) and the RBBP5–ubiquitin region (right branch). b, Overall structural comparison of human MLL1 complex with yeast COMPASS complexes. Each MLL1 component and its yeast orthologue are denoted with the same colour.
