Extended Data Fig. 6: Identification of the window of susceptibility to vitamin C deficiency between E3.5 and E13.5.
From: Maternal vitamin C regulates reprogramming of DNA methylation and germline development
a, Pregnant female mice mated in vitamin-C-deficient conditions were either maintained without vitamin C (−VitC) or returned to vitamin-C-containing water at E3.5 (3.5 return). b, Adding back vitamin C from E3.5 to E13.5 tends to rescue the defects in the expression of key germline regulators induced with full vitamin C deficiency. Gene expression was measured by qRT–PCR in E13.5 female germ cells. n = 4 biological replicates. c, The numbers of E13.5 Oct4–eGFP+ germ cells are mostly recovered after the return of vitamin C at E3.5. Data are normalized to the germ-cell count of the control embryos. n = 11–22 biological replicates of each condition as indicated. Data are mean ± s.e.m. (b, c). *P < 0.05; **P < 0.01; two-tailed Student’s t-test (b, c).
