Extended Data Fig. 4: Ripk1D325A/D325A Tnfr1−/− and Ripk1D138N, D325A/D138N, D325A mice survive until after birth.
From: Cleavage of RIPK1 by caspase-8 is crucial for limiting apoptosis and necroptosis
a, Ripk1D325A/+ Tnfr1−/− female with her 9-day-old male pups. Results representative of two Ripk1D325A/D325A Tnfr1−/− mice. b, Body weights of Ripk1D138N,D325A/D138N,D325A pups (n = 3 males, 2 females) and their littermates (n = 1 male, 4 females) aged 14–16 days. Circles, individual mice. Lines, mean ± s.e.m. P = 3 × 10−5; unpaired two-sided t-test with Welch’s correction. c, Tissues from seven-day-old littermates stained with haematoxylin and eosin. Results representative of two Ripk1D325A/D325A Tnfr1−/− and three Tnfr1−/− mice. Scale bar, 50 μm. d, Tissues from 17-day-old Ripk1D138N,D325A/D138N,D325A mice. Asterisks, granulocytic infiltrates. Results representative of two mice. Scale bar, 100 μm. e, P4–P7 offspring from intercrossing Casp8+/− mice. f, E15.5 littermates. Results representative of two mice of each genotype. g, E13.5 embryo sections immunolabelled for p-RIPK3(T231/S232) (brown). Results representative of three Casp8−/− Ripk1D138N/D138N and two Ripk1D138N/D138N embryos. Scale bar, 200 μm. h, E12.5 littermates. Results representative of four Casp8−/− Ripk1D325A/D325A and three Ripk1D325A/+ control embryos. i, Western blots of primary MEFs. β-Actin loading control performed after IκBα. For gel source data, see Supplementary Fig. 1. Results representative of two independent experiments.
