Extended Data Fig. 6: ILC3 proliferation, apoptosis and gut homing markers.
From: Light-entrained and brain-tuned circadian circuits regulate ILC3s and gut homeostasis
a, ILC3-related gene rhythmicity in small intestinal lamina propria ILC3s; n = 4. b, RNA-seq analysis of lamina propria ILC3s at ZT23; n = 3. c, Percentage of Ki67 expression in small intestine lamina propria ILC3s; n = 4. d, Percentage of donor cells in mixed bone marrow chimaeras; n = 4. e, Number of Lin−CD127+RORγt+ cells in the blood; n = 4. f, Percentage of ILC3s in mLNs. Arntlfl n = 6; ArntlΔRorgt n = 8. g, Diurnal expression of Ccr9 transcripts in gut ILC3s; n = 4. h, i, Percentage of CCR9 expression in small intestinal lamina propria CCR6−NCR−, CCR6+ (LTi-like) and NCR+ ILC3 subsets, ILC2s and CD4+ T cells; n = 3. j, Percentage of α4β7 expression in small intestine ILC1s, ILC2s and CD4+ T cells; n = 4. k, Percentage of CCR9 expression in gut ILC1s, ILC2s and CD4 + T cells; n = 4. l, m, Diurnal analysis of α4β7 and CXCR4 expression in small intestine ILC3s; n = 4. a, e, g, l, m, White, light period; grey, dark period. Data shown as mean ± s.e.m. a, c–m, n represents biologically independent animals. b, n represents biologically independent samples. a, g, Two-way ANOVA; c, d, h–k, two-tailed Mann–Whitney U test; e, l, m, cosinor regression was used to define circadian rhythmicity; f, two-tailed unpaired Student’s t-test. *P < 0.05; **P < 0.01; ***P < 0.001; NS, not significant.
