Extended Data Fig. 7: Light entrains intestinal ILC3 circadian oscillations.
From: Light-entrained and brain-tuned circadian circuits regulate ILC3s and gut homeostasis
a, Inverted feeding regimens in constant darkness. PER1–VENUS expression in gut ILC3s; n = 3. b, Circadian clock gene expression in hepatocytes of Per1Venus mice in inverted feeding regimens; n = 3. Acrophase mean ± s.e.m: Arntl: control 0.4 ± 0.5, inverted 11.5 ± 0.2; Per2: control 15.2 ± 0.6, inverted 3.9 ± 0.5; Nr1d1: control 7.1 ± 0.6; inverted 18.5 ± 0.8. c, Opposing light–dark cycles. PER1–VENUS in gut CD4−NCR−, LTi CD4+ and NCR+ ILC3 subsets; n = 3. Acrophase mean and s.e.m: CD4−NCR−: control 14.5 ± 0.5, inverted 2.5 ± 0.5; LTi CD4+: control 14.5 ± 0.6, inverted 2.5 ± 0.4; NCR+: control 14.5 ± 0.6, inverted 2.5 ± 0.4. d, PER1–VENUS MFI analysis of small intestine lamina propria ILC3s from mice maintained in constant darkness for 28 days; n = 3. b, White, light period; grey, dark period. Data are representative of three independent experiments. n represents biologically independent animals. Data shown as mean ± s.e.m. Cosinor regression. Standard errors with 95% confidence limits for amplitude (Amp) and acrophase (Acro) were extracted from the model and compared using two-tailed Student’s t-test. **P < 0.01; ***P < 0.001; NS, not significant.
