Extended Data Fig. 3: Cell-intrinsic Arntl signals control intestinal ILC3s.
From: Light-entrained and brain-tuned circadian circuits regulate ILC3s and gut homeostasis
a, Representative histogram of RFP expression in small intestine lamina propria ILC3s. Representative of three independent analyses. b, Number of Peyer patches. Arntlfl and ArntlΔVav1 n = 9; Arntlfl and ArntlΔRorgt n = 7. c, Percentage of small intestine lamina propria ILC3s; n = 5. d, Percentage of CCR6−NCR−, CCR6+ (LTi-like) and NCR+ ILC3 subsets; n = 5. e, Percentage of intestinal lamina propria CD4+ T cells and T helper (Th)17 cells; n = 5. f, Percentage and cell numbers of ILC3s, regulatory T cells (Tregs) and RORγt-expressing Tregs in the colonic lamina propria; n = 6. g, Percentage of lamina propria ILC3s, CCR6−NCR−, CCR6+ (LTi-like) and NCR+ ILC3 subsets; n = 3. h, Percentage of donor cells and cell numbers of intestinal ILC3s in mixed bone marrow chimaeras; n = 4. i, Percentage of donor cells in CCR6−NCR−, CCR6+ (LTi-like), and NCR+ ILC3 subsets of mixed bone marrow chimaeras; n = 4. j, Faecal Bacteroidetes and Firmicutes. Arntlfl n = 5; ArntlΔRorgt n = 6. j, White, light period; grey, dark period. Data are representative of at least three independent experiments. a–j, n represents biologically independent animals. Data shown as mean ± s.e.m. b–f, h–i, Two-tailed Mann–Whitney U test; j, cosinor regression was used to define circadian rhythmicity; g, two-tailed unpaired Student’s t-test. *P < 0.05; **P < 0.01; ***P < 0.001; NS, not significant.
