Extended Data Fig. 5: Network subsampling methods for preserving repertoire structure.

a, Schematic diagram of the cluster-vertex migration in the CC algorithm. b, Maximum cluster sizes between true (unsampled) networks and subsampled networks of 2,000 clones by the tree subsampling methods. c, Comparison of representative networks from each group of patients at diagnosis. The patient samples are represented across the three sampling methods. Each vertex represents a unique sequence, and the relative vertex size is proportional to the number of identical reads. Edges join vertices that differ by single nucleotide non-indel differences and clusters are collections of related, connected vertices. Networks comprise a subsample of 2,000 clones, using the corresponding subsampling method. Each vertex is represented by a pie chart that indicates the percentage of each isotype, in which blue represents IgD or IgM, red IgA1 or IgA2, yellow IgG1 or IgG2, green IgG3 and grey IgE.