Extended Data Fig. 8: A two-gene CBASS protects Bacillus against phage infection.
From: Cyclic GMP–AMP signalling protects bacteria against viral infection
a, Domain organization of a two-gene operon found in the B. cereus VD146 genome. Locus tags of the depicted genes are indicated below each gene. b, The two-gene operon from B. cereus VD146 was cloned and genomically integrated into B. subtilis BEST7003, which naturally lacks this system. The efficiency of plating of phage SBSphiC infecting the CBASS-lacking and CBASS-containing strains, as well as strains in which one of the two genes was deleted, is shown. Bar graph represents average of three independent replicates, with individual data points overlaid. c, Growth curves in liquid culture for B. subtilis containing the B. cereus two-gene CBASS, or CBASS-lacking B. subtilis that contains an empty vector instead, infected by phage SBSphiC. Bacteria were infected at time = 0 at an MOI of 0.2 or 2. Three independent replicates for each MOI are shown, and each curve shows an individual replicate.
