Extended Data Fig. 6: Cell sorting of infected cells stained with propidium iodide.
From: Cyclic GMP–AMP signalling protects bacteria against viral infection
Cells containing the CBASS derived from E. coli TW11681, and control cells containing an empty vector, were stained with propidium iodide, a fluorescent DNA-binding agent that penetrates cells that have impaired membrane integrity. Cells were infected by phage P1 (MOI of 2) and sorted on the basis of propidium-iodide fluorescence intensity (y axis); the x axis represents forward scatter. a, Uninfected cells that lack the CBASS. b, Uninfected cells that contain the CBASS. c, Cells that lack the CBASS, 40 min after infection. d, Cells that contain the CBASS, 40 min after infection. A large population of cells with high propidium-iodide fluorescence intensity is observed. Data from a representative replicate of two independent replicates are shown.
