Extended Data Fig. 3: Efficacy of antifungal treatments in pancreatic disease.
From: The fungal mycobiome promotes pancreatic oncogenesis via activation of MBL
a, Wild-type mice that bear orthotopic PDA tumours were treated with vehicle (n = 7 mice) or fluconazole (n = 8 mice), and killed three weeks later. Tumours were collected and weighed. Data are representative of experiments that were performed twice. Scale bar, 1 cm. b, Germ-free wild-type mice were treated with amphotericin B (n = 6 mice) or vehicle (n = 10 mice), and orthotopic tumours from KPC mice were administered to them. Mice were killed three weeks later, and tumours were collected and weighed. Scale bar, 1 cm. c–e, Wild-type mice induced to develop caerulein-induced pancreatitis were serially treated with amphotericin B (n = 5 mice) or vehicle (n = 3 mice). c, Representative H&E-stained sections of pancreata are shown, and pancreatic oedema was quantified by measuring the percentage of the area that was white space. Scale bar, 100 μm. d, CD45+ inflammatory cell infiltration was determined by immunohistochemistry. Scale bar, 20 μm. e, Serum levels of amylase were measured. n = 5 mice treated with amphotericin B, n = 3 mice treated with vehicle and n = 3 mock-treated (control) mice. f, Wild-type mice treated with amphotericin B were repopulated with C. tropicalis (n = 4 mice) or vehicle (n = 4 mice), and killed three weeks later. Tumours were collected and weighed. Scale bar, 1 cm. Data are mean ± s.e.m. P values determined by two-tailed Student’s t-test (a–f).
