Extended Data Fig. 4: Fungal dysbiosis drives the progression of PDA via the lectin pathway.
From: The fungal mycobiome promotes pancreatic oncogenesis via activation of MBL
a, Kaplan–Meier survival curve of patients with PDA, stratified by high (n = 16 patients), medium-high (n = 24 patients), medium-low (n = 26 patients) and low (n = 17 patients) expression of MBL on the basis of data from TCGA. b, Orthotopic tumours from KPC mice were administered to MBL-null mice treated with vehicle (n = 3 mice) or amphotericin B (n = 4 mice), and killed three weeks later. Tumours were collected and weighed. Data are representative of three separate experiments. c, MBL-null mice treated with amphotericin B were repopulated with M. globosa (n = 5 mice) or sham-repopulated (n = 4 mice), and killed three weeks later. Tumours were collected and weighed. Data are representative of experiments that were repeated twice. d, Kaplan–Meier survival curve of patients with PDA, stratified by high (n = 18) versus low (n = 15) expression of C3, on the basis of data from TCGA. e, Pancreata from three-month-old wild-type, KC and KC, MBL-null mice were stained using a monoclonal antibody against C3a. Representative images from two experiments are shown. Scale bar, 20 μm. f, KPC tumour cells were seeded in 96-well plates with vehicle or recombinant mouse C3a. n = 5 cells per group for each time point. Cellular proliferation was measured at serial time points using the XTT assay. Data are representative of experiments that were repeated three times. Data are mean ± s.e.m. P values determined by two-tailed log-rank test (a, d) or two-tailed Student’s t-test (b, c, f).
