Extended Data Fig. 7: Survival of knock-in lines on fly diets supplemented with dried, pulverized leaves of two milkweed species that host monarch butterflies in nature (A. curassavica and A. fascicularis).

a, Photograph of A. curassavica plant used in this study. b, c, Percentages of pupariating larvae and emerging adults of the knock-in and control (wild-type w¹¹¹⁸: QAN*) lines on fly diet with and without A. curassavica leaf material (n = 3–4, mean ± s.e.m.). b′, c′, Differences in pupariation and emergence percentages on a fly diet with milkweed relative to percentages on a control diet (n = 3–4, mean ± s.e.m.). Mean differences between percentages in b′ and c′ were tested with one-way ANOVA (P < 0.01) followed by post hoc Tukey’s tests (letters). These panels accompany Fig. 2e. d, Photograph of A. fascicularis plant used in this study. e, f, Percentages of pupariating larvae and emerging adults of the knock-in and control lines on fly diet with and without A. fascicularis leaf material (n = 4, mean ± s.e.m.). e′, f′, Differences in pupariation and emergence percentages on a fly diet with milkweed relative to percentages on a control diet (n = 4, mean ± s.e.m.). Mean differences between percentages in e′ and f′ were tested with one-way ANOVA (P < 0.001) followed by post hoc Tukey’s tests (letters). Experiments were performed once, and adding leaf material of either of the two milkweed species to the fly diet had largely consistent effects on survival of the monarch lineage knock-in and control fly lines.

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