Extended Data Fig. 8: The Atpα genotypes found along the monarch lineage sequentially increase TSI to ouabain without affecting baseline levels of sodium pump activity.
From: Genome editing retraces the evolution of toxin resistance in the monarch butterfly
a, In vitro ouabain sensitivity of engineered Drosophila Na+/K+-ATPases transiently expressed in Sf9 cell lines. Each data point represents the mean ± s.e.m. of three biological replicates. log10[IC50] for each type of Na+/K+-ATPase was estimated after four-parameter logistic curve fitting, and statistical differences between log10[IC50] values were tested with one-way ANOVA (P < 0.0001) followed by post hoc Tukey’s tests (letters). b, None of the sequential Atpα genotypes found along the monarch lineage affected baseline levels of pump activity in the enzymatic assay with extracts of transiently transfected Sf9 cells (one-way ANOVA, P = 0.3197). Each data point represents the mean ± s.e.m. of three biological replicates.
