Extended Data Fig. 4: Drosophila flies with edited genomes show increased larval–adult survival when fed cardiac glycosides.
From: Genome editing retraces the evolution of toxin resistance in the monarch butterfly
These panels accompany Fig. 2c. Larval–adult survival when reared on diets with a range of ouabain concentrations was different between monarch lineage knock-in lines relative to control lines (QAN, engineered control; QAN*, w1118 wild type). Symbols represent the mean ± s.e.m. of 3–6 biological replicates (50 larvae per replicate). Curves were fit through a univariate logistic regression (effect of ouabain concentration on survival), and the difference in survivorship trajectories between each pair of fly lines (genotypes) was evaluated by performing an LRT to assess the significance of the inclusion of an interaction term between genotype and ouabain concentration in the logistic regression for a pair of lines (**P < 0.01, ***P < 0.001). Further information on experiment design and statistical test results is in the Source Data.
