Extended Data Fig. 2: Spatiotemporal dynamics of naive CD8+ T cells after intrahepatic priming.
From: Dynamics and genomic landscape of CD8+ T cells undergoing hepatic priming
Five million fluorescent Cor93 TN cells were transferred into MUP-core mice or wild-type mice transduced with rLCMV-core. Mice were splenectomized and treated with anti-CD62L antibody 48 h or 4 h before Cor93 TN transfer cell, respectively. a, Left, confocal immunofluorescence micrographs of liver sections from the indicated mice at the indicated time points after Cor93 TN cell transfer, showing the distribution of Cor93 T cells (green) relative to portal tracts (highlighted by anti-cytokeratin 7 (CK-7)-antibody-mediated staining of bile ducts in red). Sinusoids are highlighted by anti-LYVE-1+ antibodies (white). Scale bars, 100 μm. Right, immunohistochemical micrographs of liver sections from the indicated mice at the indicated time points after Cor93 TN cell transfer, showing the distribution of leukocyte infiltrates relative to portal tracts (highlighted by anti-CK-7-antibody-mediated staining of bile ducts in brown). Scale bars, 100 μm. b, Distribution of the distances of each Cor93 T cell from the centre of the closest portal triad at the indicated time points. n = 3 mice. Data are representative of at least three independent experiments.
