Extended Data Fig. 9: Swi6–chromatin assemblies present liquid-like properties.
From: HP1 reshapes nucleosome core to promote phase separation of heterochromatin
a, Chromatin–Swi6 condensates are reversible after dilution. b, Chromatin–Swi6 droplets fuse within seconds after incubation. The time course depicted is 30 s. c, Chromatin is included in Swi6-induced condensates. Nucleosome arrays are assembled with H2A–Cy3 or H2A–Cy5 and H3Kc9me3 octamers. Images showing Cy3- or Cy5-labelled chromatin mixed with Swi6 separately, before being incubated together. d, Images show condensates 30 min after mixing Cy3 and Cy5 pre-formed droplets. By around 1 h, most of the droplets contain homogeneous labelling with both dyes. Doubly labelled droplets indicate chromatin exchanges between the pre-formed condensates. e, Quantification of fluorescence intensity done in ImageJ using the Plot Profile function that creates a plot of intensity values across the yellow line shown in the left panel. Higher octamer concentration within the droplets as compare to the bulk of the solution is detected. Images are representative images from at least three independent experiments.
