Extended Data Fig. 1: Swi6 interaction with chromatin.
From: HP1 reshapes nucleosome core to promote phase separation of heterochromatin
a, Model for HP1-mediated heterochromatin assembly: the bridging model relies on the ability of HP1 to oligomerize across nucleosomes; the phase separation model relies on the ability of HP1 to form phase-separated assemblies that can sequester chromatin. The molecular link between these two models is unclear. b, Sedimentation velocity-analytical ultracentrifugation (SV-AUC) analysis on H3Kc9me3 dinucleosomes. The continuous sedimentation coefficient distribution (c(s)) is shown as a two-dimensional distribution. The measured masses using a continuous function c(s) distribution versus theoretically predicted masses are shown. c, SV-AUC on H3Kc9me3 dinucleosomes with Swi6. Data are as in b. Analysis is performed using a continuous function c(s) with a bimodal f/f0 distribution. The peak at s = 21.24 corresponds to the Swi6–dinucleosome complex. The peak at s = 4.5 corresponds to free Swi6 dimers, as previously shown4. d, e, Analysis of raw SV-AUC data for dinucleosome alone (d) and dinucleosome plus Swi6 complex (e) showing that: (i) the fit lines describe the boundary data well; (ii) the r.m.s.d. value is below 0.01; and (iii) the residual bitmap has very few diagonal features indicating the good quality of the fit. For b–d, measurements are representative of two independent experiments.
