Extended Data Fig. 5: Nutrient-dependent plasticity does not occur in the majority of Crz neurons except a pair of the dorsolateral CN neurons.
From: A glucose-sensing neuron pair regulates insulin and glucagon in Drosophila
a–d, The relative fluorescence intensities of Syt–GFP signals (a, b) and the branch lengths (c, d) of axon 1 (A1) (neurite to IPCs, a, c) and axon 2 (A2) (neurite to CC, b, d) in fed, starved or refed flies carrying Crz-Gal4 and UAS-Syt::GFP. e–h, Representative images (e) and quantifications (f–h) of the number of Syt–GFP+ puncta in fed, starved or refed flies carrying Crz-Gal4 and UAS-Syt::GFP in the dorsal lateral area (DL, f), the medial lateral area (ML, g) and the SEZ. In h, Arrowheads and arrows indicate Syt–GFP+ puncta on A1 and A2, respectively. Scale bar, 50 µm. Images are z-stacked projections. *P < 0.05, **P < 0.01 and ***P < 0.001; one-way ANOVA with Tukey post hoc test. See Supplementary Table 1 for the sample sizes and statistical analyses.
