Extended Data Fig. 6: CN neurons expressing P2X2 receptor can be readily activated by ATP application, and Dh44 neurons and CN neurons are not functionally coupled.
From: A glucose-sensing neuron pair regulates insulin and glucagon in Drosophila
a, b, Average GCaMP traces (a) and their ΔF/F (max) quantifications (b) of CN neurons when exposed to 2.5 mM ATP. c, Average GCaMP trace of flies carrying Dh44-Gal4, UAS-GCaMP6s in response to ATP. d, Average GCaMP trace of flies carrying Dh44-Gal4, UAS-GCaMP6s, Dh44-LexA and LexAop-P2X2 in response to ATP. e, Average GCaMP trace of flies carrying Crz-Gal4, UAS-GCaMP6s, Dh44-LexA and LexAop-P2X2 in response to ATP. f, Average GCaMP trace of flies carrying Dh44-Gal4, UAS-GCaMP6s, R20F11-LexA and LexAop-P2X2 in response to ATP. ***P < 0.001; one-way ANOVA with Tukey post hoc test. See Supplementary Table 1 for the sample sizes and statistical analyses.
