Extended Data Fig. 3: The activity of CN neurons is stimulated by glucose or pyruvate, but not by sucrose or a mixture of essential amino acids.
From: A glucose-sensing neuron pair regulates insulin and glucagon in Drosophila
a, Representative traces of CN neuronal activity in response to 2 mM, 10 mM and 15 mM d-glucose. b–e, Quantifications of CN responses to 1 mM, 2 mM, 5 mM, 10 mM, 15 mM and 20 mM d-glucose. When 1 to 5 mM d-glucose were applied, all parameters increased. When higher concentrations (10 to 20 mM d-glucose) were applied, the oscillation number (c) and oscillation frequency (e) decreased, while the peak amplitude (b) and duration (d) increased. f, Representative traces of CN neuronal activity in response to 20 mM sucrose, 20 mM pyruvate and a mixture of ten EAAs. g–j, Quantifications of CN neuronal responses to 20 mM sucrose, 20 mM pyruvate and a mixture of 10 EAAs. *P < 0.05 and **P < 0.01; one-way ANOVA with Tukey post hoc. See Supplementary Table 1 for the sample sizes and statistical analyses.
