Extended Data Fig. 5: FSP1 protects against lipid peroxidation by reducing radical-trapping antioxidants.
From: FSP1 is a glutathione-independent ferroptosis suppressor
a, b, Co-autoxidations of STY-BODIPY (1 μM) (a) and the polyunsaturated lipids of chicken egg phosphatidylcholine liposomes (1 mM). The increase in fluorescence of oxidized STY-BODIPY is monitored over the course of the autoxidation, which is initiated using C9-HN (0.2 mM) (b). c, Representative autoxidations inhibited by 50 nM FSP1 (green), 8 μM NADH (purple), 16 μM NADH (orange), 50 nM FSP1 and 8 μM NADH (black) or 50 nM FSP1 and 16 μM NADH (blue) (c). d–j, Analogous representative of inhibited autoxidations to which CoQ10 (d, e), α-tocopherol (α-tocopherol) and CoQ10 (h, i), or α-tocopherol (j) was added. f, g, Recombinant NQO1 failed to suppress autoxidations in a similar manner compared to FSP1 (f, g). k, 1-Palmitoyl-2-linoleoyl-phosphatidylcholine hydroperoxide (PLPC-OOH) produced from the autoxidation of soy lecithin liposomes (13.3 mM), inhibited by FSP1 alone, or in the presence of either 10 μM CoQ10 or 10 μM α-tocopherol and 10 μM CoQ10. PLPC-OOH was measured 0, 60, 120 and 180 min after autoxidation was induced using liquid chromatography–mass spectrometry (MRM: 790 → 184). Data show one of n = 3 representative experiments.
