Extended Data Fig. 4: Expressing ND1 in mouse L3161C lung ADC cell line reduces sensitivity of mitochondrial membrane potential to phenformin in vitro and in vivo.
From: In vivo imaging of mitochondrial membrane potential in non-small-cell lung cancer
a, Basal OCR rate per cell for L3161C cells expressing empty vector (pBabe; black) (n = 12 technical replicates) or L3161C cells expressing ND1 (L3161C-ND1; red) (n = 12 technical replicates) treated with 50 μM phenformin for 24 h. b, Basal OCR rate per cell for L3161C-pBabe (black) (n = 12 technical replicates for all conditions, except n = 6 for 250 μM and n = 9 for 500 μM phenformin) and L3161C-ND1 cells (red) (n = 12 technical replicates) treated with the indicated concentrations of phenformin for 24 h. Data are mean ± s.d. c, Waterfall plot of the percentage change in maximum uptake of 18F-BnTP after treatment relative to before treatment for mice transthoracically implanted with L3161C cells expressing empty vector (pBabe; n = 5 mice) or ND1 (n = 5 mice) and treated with 125 mg kg−1 phenformin for 5 days. d, Waterfall plot of the percentage change in maximum uptake of 18F-BnTP in tumours formed by transthoracically implanted L3161C-pBabe (n = 3 mice) or L3161C-ND1 cells (n = 5 mice) treated with vehicle for 5 days. Experiments in a–d were performed once. P values determined by unpaired two-tailed t-test.
