Extended Data Fig. 6: Iα deletion increases iEμ–Iμ and HS4 interactions across the upstream CH domain.
From: Fundamental roles of chromatin loop extrusion in antibody class switching
a, b, Left, additional repeats for the 3C-HTGTS profiles shown in Fig. 2d, e, from anti-CD40–IL-4–TGFβ-stimulated CH12F3NCΔ AID−/− and IαΔ AID−/− cells using iEμ–Iμ (a) or HS4 (b) locale as baits (blue asterisks). Green asterisks indicate 3′ IgH RR HS sites in all panels. Grey bars highlight the iEμ–Cμ, Sγ3, Sγ2b, Sγ2a, Sα, 3′ IgHRR and 3′ CBEs. Right, magnified view of the 3C-HTGTS profiles on the left to better reveal the interaction patterns in the region from Iγ3 to Cε in anti-CD40–IL-4–TGFβ-stimulated CH12F3NCΔ AID−/− and IαΔ AID−/− cells. c, Left, 3C-HTGTS profiles of interactions across the indicated domain of non-stimulated and anti-CD40–IL-4–TGFβ-stimulated IαΔ AID−/− cells using the iEμ–Iμ locale as bait (blue asterisks). Grey bars highlight the iEμ–Cμ, Sγ3, Sγ2b, Sγ2a, Sα, 3′ IgHRR and 3′ CBEs. Right, magnified view of the 3C-HTGTS profiles on the left to better reveal the interaction patterns in the region from Sγ3 to Sε in non-stimulated and anti-CD40–IL-4–TGFβ-stimulated IαΔ AID−/− cells.
