Extended Data Fig. 9: Validation of degenerate bhcC primers. | Nature

Extended Data Fig. 9: Validation of degenerate bhcC primers.

From: Marine Proteobacteria metabolize glycolate via the β-hydroxyaspartate cycle

Extended Data Fig. 9

Degenerate primers for bhcC were used for qPCR with different amounts of genomic DNA from P. denitrificans DSM 413, Rhodobacter sphaeroides 2.4.1, and E. coli K-12 MG1655 as template. While the bhcC gene from P. denitrificans DSM 413 is amplified, genomic DNA from organisms that lack the bhc gene cluster does not result in reliable amplification. Data are mean ± s.d.; n = 3 independent experiments.

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