Extended Data Fig. 3: Transmembrane proton transport.
From: Single-chain heteropolymers transport protons selectively and rapidly
a, pH gradient-driven proton flux through RHP1 in the LUVs of POPE/POPG (molar ratio 3:1). b, Inner pH changes in POPC LUVs incubated with RHP1 and RHP variants over 200 s after adding Vln. ‘RHPwoM’, ‘RHPwoSP’ and ‘RHP7/0’ represent the RHP without MMA, the RHP without SPMA and the RHP without EHMA, respectively. c, pH gradient-driven proton flux in the influx and outflux directions. RHP1 was added to the solutions of preformed LUVs. d, pH gradient-driven proton flux through RHP1 in DMPC SUVs at 37 °C. RHP1-containing SUVs were prepared as indicated in the Methods section ‘SANS investigation’ using H2O-based buffers, with an RHP1:DMPC ratio of 0.001.
