Extended Data Fig. 5: SANS investigations.
From: Single-chain heteropolymers transport protons selectively and rapidly
a, SANS profiles of free RHP1 in 86% D2O. The solid line denotes a fit using a polydisperse sphere model for RHP1. The signal of the solvent has been subtracted in the presented curves. b, Schematic illustration of the three-shell vesicle model for the SUV, showing the regions of the liposome core (core), inner bilayer headgroup (shell 1), lipid bilayer core (shell 2) and outer bilayer headgroup (shell 3). c, Structural parameters obtained by fitting the SANS data with the three-shell vesicle model for the liposome and the polydisperse sphere model for RHP1. Parameters that were fixed during the fitting are denoted with ‘f’; RMS represents root mean square. SLDs that were fixed were determined from the known sample composition, including when RHP1 was present in the bilayer, because the concentration was too low to impact the SLD value of shells significantly. The SLD values used for the liposome core, for shells 1, 2, 3 and for the solvent are 5.41, 1.88, 6.84, 1.88 and 5.41 × 10−6 Å−2, respectively. The SLD value for RHP1 is 1.4 × 10−6 Å−2 in the sphere model.
