Extended Data Fig. 6: Glucose, insulin and GDF15 response to metformin.
From: GDF15 mediates the effects of metformin on body weight and energy balance
a, Fasting glucose from oral GTT as in Fig. 3e, f. ANOVA; effect of antibody, P = 0.028; effect of metformin, P = 0.271; interaction of antibody and metformin, P = 0.707. b, Circulating GDF15 in mice undergoing intraperitoneal GTT after a single dose of metformin as in Fig. 3 k, l. P values by two-way ANOVA with Tukey’s correction for multiple comparisons. c, d, Fasting glucose (c) and fasting insulin (d) at time 0 of intraperitoneal GTT as in Fig. 3k, l; not statistically significant by two-way ANOVA. e, AUC analysis of glucose levels as in Fig. 3k, l. P values by two-way ANOVA, effect of genotype, P = 0.392; interaction of genotype and metformin, P = 0.883. a–e, Data all mean ± s.e.m. f, Circulating GDF15 levels in high-fat-diet-fed Gdf15+/+ mice after single oral dose of metformin (600 mg kg−1). Samples were collected 6 h after dosing, data are mean ± s.e.m., n = 7 per group; P values (95% CI) by two-tailed t-test.
