Extended Data Fig. 7: In situ hybrididation for Gdf15 mRNA expression in gut, liver and kidney.
From: GDF15 mediates the effects of metformin on body weight and energy balance
a, Representative images from the mouse with circulating GDF15 level closest to the group median shown in Fig. 4b, with images from other regions of the gut and from liver. b, In situ hybridization for Gdf15 mRNA expression (red spots) in colon. Tissue collected from high-fat-diet-fed wild-type mice, 6 h after a single dose of oral metformin (600 mg kg−1) (right, red box, M1–M7) or vehicle gavage (left, blue box, V1–V7); n = 7 mice per group, mice as in Fig. 4.
