Extended Data Fig. 3: Body weight changes with metformin treatment in mice with disrupted GDF15–GFRAL signalling.
From: GDF15 mediates the effects of metformin on body weight and energy balance
a, Absolute body weight in Gdf15+/+ and Gdf15−/− mice on a high-fat diet treated with metformin (300 mg kg−1 day−1) for 11 days, mice as in Fig. 2a. Data are mean ± s.e.m., P values by two-way ANOVA with Tukey’s correction for multiple comparisons. b, Absolute body weight in high-fat diet-fed Gfral+/+ and Gfral−/− mice given an oral dose of metformin (300 mg kg−1) once daily for 11 days, mice as in Fig. 2c. Data are mean ± s.e.m. c, Absolute body weight of metformin-treated, obese mice dosed with an anti-GFRAL antagonist antibody or with control IgG weekly for five weeks, starting four weeks after initial metformin exposure; mice as in Fig. 2d. Data are mean ± s.e.m. P values by two-way ANOVA with Tukey’s correction for multiple comparisons.
