Extended Data Fig. 8: Flow cytometry analysis of myeloid cell populations after treatment with VEGF-C.
From: VEGF-C-driven lymphatic drainage enables immunosurveillance of brain tumours
Mice bearing 7-day tumours were treated with Luc mRNA or VEGFC mRNA and evaluated for changes in the populations of myeloid cells. a, Gating strategy for different myeloid cells. b–d, Cell counts of different cell types were measured at different time points after VEGFC mRNA treatment. cDC, classical dendritic cell; DC, dendritic cell; pDC, plasmacytoid dendritic cell. e–g, MHC II and CD80 MFI levels were quantified and showed no significant alteration after treatment with VEGFC mRNA. b and e are leukocytes from brain tissue; c and f are leukocytes from draining cervical lymph nodes; d and g are leukocytes from meninges (n = 3, 3 mice were pooled for each replicate). Data are mean ± s.e.m *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001 (two-tailed unpaired Student’s t-test).
