Extended Data Fig. 7: Plasticity in patterns of PKA activity in SPNs during learning.
From: Cell-type-specific asynchronous modulation of PKA by dopamine in learning
a, Heat maps of SPN FLIM-AKAR response for success trials during learning. Each row represents an individual trial. Red lines or dots indicate behavioural time stamps (T = trigger zone entry, L = LED on, Z = receptacle zone entry, D = pellet dispensing, R = receptacle entry). Different colours in mouse ID columns represent different mice for an individual row. Top, D1R-SPN FLIM-AKAR responses of Drd1a-cre mice. n = 98 trials (beginner), 418 trials (intermediate), 873 trials (expert) from 14 mice. Bottom, D2R-SPN FLIM-AKAR responses of Adora2a-cre mice. n = 134 trials (beginner), 596 trials (intermediate), 1,152 trials (expert) from 18 mice. b, Heat maps of SPN FLIM-AKAR response for failure trials during learning. Plotted as in a. Top, D1R-SPN FLIM-AKAR responses of Drd1a-cre mice. n = 497 trials (beginner), 402 trials (intermediate), 122 trials (expert) from 14 mice. Bottom, D2R-SPN FLIM-AKAR responses of Adora2a-cre mice. n = 528 trials (beginner), 416 trials (intermediate), 218 trials (expert) from 18 mice. c, Heat maps of SPN FLIM-AKAR response for reward-omission trials (left) and rewarded LED-omission trials (right). Plotted as in a. Top, D1R-SPN FLIM-AKAR responses of Drd1a-cre mice. n = 69 trials (reward omission) from 14 mice, 25 trials (rewarded LED-omission trials) from 6 mice. Bottom, D2R-SPN FLIM-AKAR responses of Adora2a-cre mice. n = 91 trials (reward omission) from 18 mice, 35 trials (rewarded LED-omission trials) from 10 mice.
