Extended Data Fig. 10: OsTCP19-H increases crop yield and NUE of modern cultivars.
From: Genomic basis of geographical adaptation to soil nitrogen in rice
a, b, Statistical analysis of tiller number (a) (n = 22 plants) and grain yield per plant (b) (n = 20 plants) of Kos and NILOsTCP19-H plants under two nitrogen conditions in Beijing in 2017. c, Phenotype of Kos and NILOsTCP19-H plants grown under two nitrogen conditions in Beijing in 2018. Scale bar, 24 cm. d–g, Statistical analysis of Kos and NILOsTCP19-H plants grown under two nitrogen conditions in field tests in Beijing in 2018. Tiller number (d) (n = 54 plants), grain yield per plant (e) (n = 20 plants), plot yield (f) (n = 4 plots) and NUE (g) (n = 4 plots) of Kos and NILOsTCP19-H plants under two nitrogen conditions. h, Phenotype of Kongyu131 (KY131) and KY131OsTCP19-H plants under two nitrogen conditions. Scale bar, 24 cm. i, Statistical analysis of tiller number shown in h (n = 50 plants). j, Phenotype of Xiushui134 (XS134) and XS134OsTCP19-H plants under two nitrogen conditions. Scale bar, 24 cm. k, Statistical analysis of tiller number shown in j (n = 50 plants). l, A heat map of DEGs involved in nitrogen metabolism pathway (Kyoto Encyclopedia of Genes and Genomes, ko00910). The colour key (red to yellow) represents gene expression (FPKM) as log2-transformed fold changes of (TO1/WT) or (TO2/WT). The gene-encoding proteins are shown on the right. m, qRT–PCR-based transcript abundance analysis of OsNRT2.1, OsNRT2.2, OsAMT1.3, OsNIR1 and OsGS1.2 in Kos and NILOsTCP19-H plants. Data are mean ± s.d. (n = 3 biologically independent samples). n, 15N accumulation in roots of Kos and NILOsTCP19-H plants. 15N-nitrate and 15N-ammonium were used for 15N labelling in Kos and NILOsTCP19-H plants, respectively. DW, dry weight. Data are mean ± s.d. (n = 4 biologically independent samples). In a, b, d, e, i, k, the bars in the violin plots represent 25th percentiles, medians, and 75th percentiles. f, g, The horizontal bars of boxes represent minima, 25th percentiles, medians, 75th percentiles and maxima. In a, b, d–g, i, k, different letters indicate significant differences at P < 0.05 according to one-way ANOVA and Tukey’s HSD test. For P values, see Source Data. In m, n, significant difference was determined by the two-sided Student’s t-test.
