Extended Data Fig. 4: OsTCP19 acts as a negative modulator in rice tillering and TRN.
From: Genomic basis of geographical adaptation to soil nitrogen in rice
a, The neighbour-joining tree was constructed in MEGA 5.0. The numbers represent bootstrap (1,000 replicates). OsTCP19 and its orthologues in Arabidopsis are shown in red text. b, Phenotype of ZH11 and cTO lines (cTO1 and cTO2) under low nitrogen (50 kg ha−1) and moderate nitrogen (150 kg ha−1) conditions. Scale bar, 24 cm. c, Expression analysis of OsTCP19 in ZH11, cTO1 and cTO2 lines by qRT–PCR. Data are mean ± s.d. (n = 3 biologically independent samples). d, Statistical analysis of tiller number per plant of ZH11, cTO1 and cTO2 lines under low and moderate nitrogen conditions. Data are mean ± s.e.m. (n = 24 plants). e, TRN of ZH11, cTO1 and cTO2 lines generated from d. Data are mean ± s.e.m. (n = 24 plants). f, Phenotype of ZH11, T-Ri1 and T-Ri2. Scale bar, 24 cm. g, qRT–PCR analysis of OsTCP19 expression level in ZH11, T-Ri1 and T-Ri2 lines. Data are mean ± s.d. (n = 3 biologically independent samples). h, Statistical analysis of tiller number per plant of ZH11, T-Ri1 and T-Ri2 lines. Data are mean ± s.e.m. (n = 18 plants). i, Diagram of OsTCP19 CRISPR knockout lines (T-cr1 and T-cr2). The length and the position of the mutations are indicated on the frame. In c, d, e, g, h, different letters indicate significant differences (P < 0.05, one-way ANOVA, Tukey’s HSD test). For P values, see Source Data.
