Extended Data Fig. 8: Longitudinal Env amino acid divergence from the input virus and relationship with viral load in the plasma.
From: Robust and persistent reactivation of SIV and HIV by N-803 and depletion of CD8+ cells
The number of amino acid differences between the infecting viral clone SIVmac239 and each SGA amplicon was determined using Geneious. a, Violin plots show the frequency distribution of the number of amino acid differences between sequences at each time point in each macaque. The solid line indicates the median number of amino acid differences for each individual Env sequence; the dotted lines indicate the quartiles. Peak viral load (VL) (red), pre-ART (blue) and reactivation (green) time points are shown. The animal ID and the three time points are indicated on the x axis. Statistical differences between time points for each macaque were determined by performing multiple comparisons using a Kruskal–Wallis test with Dunn’s correction. b, The average number of sequence differences for each macaque at the reactivation time point is plotted on the y axis, and the corresponding plasma viral loads are plotted on the x axis on a log10 scale. Correlation coefficients are calculated using the Spearman’s rank-order correlation (two-tailed, no adjustments).
