Extended Data Fig. 1: MT807R1 effectively depletes CD8⁺ T cells in peripheral blood, lymph node and rectum of macaques in addition to depleting NK cells from the blood at day 7.

The percentage of CD8⁺ cells in the CD3⁺ population 7 days after depletion was compared to the levels before depletion. a–c, Sample flow cytometry shows the absence of CD8β⁺ cells as part of the CD3⁺ T cell population after depletion in the peripheral blood (a), rectum (b) and lymph nodes (c) of macaques. Similar results were found across all CD8-depleted macaques (n = 28 biologically independent samples). d, The percentage of CD8β⁺ cells compared to pre-depletion baseline was calculated in all CD8-depleted macaques (treated with or without N-803, n = 28 macaques) across blood and tissue samples (no differences in CD8⁺ T cell depletion were observed between groups 2 and 3 on day 7). A two-sided Friedman test was used to calculate statistically significant changes compared to baseline across tissues. e, Depletion of NK cells in the peripheral blood was assessed 1 week after CD8 depletion alone (n = 14 macaques) compared to baseline. Statistical significance was calculated using Wilcoxon signed-rank test. Data are mean ± s.e.m.